Research Interest

Prevention of allergy development by early postnatal probiotic supplementation

Collaborations

University of Alabama at Birmingham
Alabama, USA
prof. Zdenek Hel

Lactic Acid Bacteria and Mucosal Immunity, Centre of Infection and Immunity Lille

Institute Pasteur, Lille, France
dr. Corinne Grangette, dr. Catherine Daniel, dr. Mathias Chamaillard

Instituto de Lactología Industrial, Facultad de Ingeniería Industrial Química
Sante Fe, Argentina
prof. Gabriel Vinderola
prof. Patricia Burns

Institute of Immunology and Experimental Therapy
Polish Academy of Science
Poland
dr. Sabina Gorska

Medical University of Vienna, Austria
assoc. prof. Irma Schabussova

Universidade Federal Fluminense, Niterói
Brazil
Isabelle Guimaraes

Ongoing GRANTs

2025-2028
NW25-05-00411

Identification of new targets for diagnosis, prevention and therapy of non-IgE food allergies

Identifikace nových cílů pro diagnostiku, prevenci a terapii non-IgE potravinových alergií

2025-2027
GAČR 16064S

Borrelia elimination in ticks: unveiling vaccine mechanisms

Eliminace borelií v klíšťatech: objasnění vakcinačních mechanismů

2023-2026
MPO/EI

Verification of the positive effect of the unique probiotic strain Escherichia coli O83 for the treatment and prevention of severe intestinal diseases in humans on animal models (EcO83)

2023-2026
AZV ČR

Altered proportional and functional characteristics of particular neutrophil subsets as a possible trigger of relapses in patients with autoimmune neurological disorders of CNS

2022-2026
Cooperatio IMMU207032

Allergology and Clinical Immunology

2023-2024
MSM 8J23AT019

Characterization of immunomodulatory properties of extracellular vesicles derived from probiotic bacteria and their migration in the host

2023-2024

MSM 8J23FR003

The capacity of E. coli probiotic strain to persist in the murine gut and to ameliorate colitis via autophagy induction

2022-2024

GAČR

Effect of larval cestodes on cancers and malignant cells in mice

Publications

Švubová, V., Janstová, L., Jedlička, M., Mašínová, E., Szabová, J., Feglarová, T., ... & Frič, J. (2025). TGF-β Decreases NK Cell Mobility and Cytotoxic Efficacy in Complex in vitro Models of the Leukemia Microenvironment. ImmunoTargets and Therapy, 589-604.

Koucky, M., Lastuvka, Z., Koprivova, H., Cindrova-Davies, T., Hrdy, J., Cerna, K., Calda, P. (2025) Reduced number of regulatory T cells in maternal circulation precede idiopathic spontaneous preterm labor in a subset of patients. Am J Obstet Gynecol, 232(2), 222.e1-222.e11

Razim, A., Zabłocka, A., Schmid, A., Thaler, M., Černý, V., Weinmayer, T., ... & Schabussova, I. (2024). Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells. Journal of Extracellular Vesicles, 13(10), e70004.

Miková, E., Černý, V., Novotná, O., Petrásková, P., Boráková, K., Hel, Z., & Hrdý, J. (2024). Immature neutrophils in cord blood exert increased expression of genes associated with antimicrobial function. Frontiers in Immunology, 15, 1368624.

Schreiber, M., Macháček, T., Vajs, V., Šmídová, B., Majer, M., Hrdý, J., ... & Horák, P. (2024). Suppression of the growth and metastasis of mouse melanoma by Taenia crassiceps and Mesocestoides corti tapeworms. Frontiers in Immunology, 15, 1376907.

Welsh, J. A., Goberdhan, D. C., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., ... & Benedikter, B. J. (2024). Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Journal of extracellular vesicles, 13(2), e12404.

Schmid, A. M., Razim, A., Wysmołek, M., Kerekes, D., Haunstetter, M., Kohl, P., ... & Schabussova, I. (2023). Extracellular vesicles of the probiotic bacteria E. coli O83 activate innate immunity and prevent allergy in mice. Cell Communication and Signaling, 21(1), 297.

Černý V, Hrdý J, Beneš J, Tlaskalová H. (2023) A word on the microbiome: considerations about the history, current state, and terminology of an emerging discipline. Epidemiol Mikrobiol Imunol, 72(2), 99-105.

Nytrova P, Stastna D, Tesar A, Menkyova I, Posova H, Koprivova H, Mikulova V, Hrdy J, Smela G, Horakova D, Rysankova I, Doleckova K, Tyblova M. (2023) Immunity following SARS-CoV-2 vaccination in autoimmune neurological disorders treated with rituximab or ocrelizumab. Frontiers in Immunology, 14, 1148629.

Súkeníková L, Černý V, Thon T, Roubalová R, Jirásková Zákostelecká Z, Novotná O, Petrásková P, Boráková K, Kocourková I, Lodinová-Žádníková R, Musil Z, Kolářová L, Prokešová L, Valenta Z, Hrdý J. (2023) Effect of early postnatal supplementation of newborns with probiotic strain E. coli O83:K24:H31 on allergy incidence, dendritic cells, and microbiota. Frontiers in Immunology, 13, 1038328.

Súkeníková L, Černý V, Věcek J, Petrásková P, Novotná O, Vobruba Š, Procházka J, Michalčíková T, Kolářová L, Prokešová L, Hrdý J. (2022) The impact of Escherichia coli probiotic strain O83:K24:H31 on the maturation of dendritic cells and immunoregulatory functions in vitro and in vivo. Cells, 11(10), 1624.

Novák J, Macháček T, Majer M, Kostelanská M, Skulinová K, Černý V, Kolářová L, Hrdý J, Horák P. (2022) Toxocara canis infection worsens the course of experimental autoimmune encephalomyelitis in mice. Parasitology, 1-9.

Hrdý J, Couturier-Maillard A, Boutillier D, Lapadatescu C, Blanc P, Pot B, Procházka J, Ryffel B, Grangette C, Chamaillard M. (2022) Oral supplementation with selected Lactobacillus acidophilus triggers IL-17-dependent innate defense response, activation of innate lymphoid cells type 3 and improves colitis. Scientific Reports, 12(1), 17591.

Golshani, M., & Hrdý, J. (2022). Multiple Sclerosis Patients and Disease Modifying Therapies: Impact on Immune Responses against COVID-19 and SARS-CoV-2 Vaccination. Vaccines, 10(2), 279.

Černý, V., Novotná, O., Petrásková, P., Hudcová, K., Boráková, K., Prokešová, L., ... & Hrdý, J. (2021). Lower Functional and Proportional Characteristics of Cord Blood Treg of Male Newborns Compared with Female Newborns. Biomedicines, 9(2), 170.

Černý, V., Petrásková, P., Novotná, O., Boráková, K., Prokešová, L., Kolářová, L., & Hrdý, J. (2020). Value of cord blood Treg population properties and function-associated characteristics for predicting allergy development in childhood. Central-European Journal of Immunology, 45(4), 393.

Herbrík, A., Corretto, E., Chroňáková, A., Langhansová, H., Petrásková, P., Hrdý, J., ... & Petříčková, K. (2020). A human lung-associated streptomyces sp. TR1341 produces various secondary metabolites responsible for virulence, cytotoxicity and modulation of immune response. Frontiers in Microbiology, 10, 3028.

Cuffaro, B., Assohoun, A. L., Boutillier, D., Súkeníková, L., Desramaut, J., Boudebbouze, S., ... & Grangette, C. (2020). In vitro characterization of gut microbiota-derived commensal strains: selection of Parabacteroides distasonis strains alleviating TNBS-induced colitis in mice. Cells, 9(9), 2104.

Miková, E., Hrdý, J. (2020). The role of neutrophils in preeclampsia. Ceska Gynekol, 85(3), 206.

Hrdý, J., Súkeníková, L., Petrásková, P., Novotná, O., Kahoun, D., Petříček, M., ... & Petříčková, K. (2020). Inhibition of Pro-Inflammatory Cytokines by Metabolites of Streptomycetes—A Potential Alternative to Current Anti-Inflammatory Drugs? Microorganisms, 8(5), 621.

Majer, M., Macháček, T., Súkeníková, L., Hrdý, J., & Horák, P. (2020). The peripheral immune response of mice infected with a neuropathogenic schistosome. Parasite Immunology, 42(6), e12710.

Hrdý, J., Alard, J., Couturier-Maillard, A., Boulard, O., Boutillier, D., Delacre, M., ... & Grangette, C. (2020). Lactobacillus reuteri 5454 and Bifidobacterium animalis ssp. lactis 5764 improve colitis while differentially impacting dendritic cells maturation and antimicrobial responses. Scientific Reports, 10(1), 1-11.

Tlaskalová-Hogenová, H., Kverka, M., & Hrdý, J. (2020). Immunomodulatory components of human colostrum and milk. Milk, Mucosal Immunity and the Microbiome: Impact on the Neonate, 94, 38-47.

Lohonková, A., Novotná, O., Petrásková, P., Boráková, K., Prokešová, L., & Hrdý, J. (2019). Maternal Allergy Status Has No Impact on Neonatal Immune Responses to Allergen Stimuli. Folia Biologica, 65(5/6), 221-226.

Hrdý, J., Novotná, O., Petrásková, P., Boráková, K., & Prokešová, L. (2019). Different immune response of dendritic cells of newborns of allergic and healthy mothers to bacterial stimuli. Folia Microbiologica, 64(6), 797-802.

Lambert, L., Novakova, M., Lukac, P., Cechova, D., Sukenikova, L., Hrdy, J., ... & Grus, T. (2019). Evaluation of the immunogenicity of a vascular graft covered with collagen derived from the European carp (Cyprinus carpio) and bovine collagen. BioMed Research International.

Hrdý J, Novotná O, Petrásková P, Boráková K, Lodinová-Žádníková R, Prokešová, L. (2019) Vliv časného postnatálního podání probiotického kmene Escherichia coli O83:K24:H31 na rozvoj alergických onemocnění a vybrané imunologické charakteristiky. Alergie, 21(1) 17-22.

Černý, V., Hrdý, J., Novotná, O., Petrásková, P., Boráková, K., Kolářová, L., & Prokešová, L. (2018). Distinct characteristics of Tregs of newborns of healthy and allergic mothers. PLoS One, 13(11), e0207998.

Hrdý, J., Vlasáková, K., Černý, V., Súkeníková, L., Novotná, O., Petrásková, P., ... & Prokešová, L. (2018). Decreased allergy incidence in children supplemented with E. coli O83: K24: H31 and its possible modes of action. European Journal of Immunology, 48(12), 2015-2030.

Macháček, T., Turjanicová, L., Bulantová, J., Hrdý, J., Horák, P., & Mikeš, L. (2018). Cercarial dermatitis: a systematic follow-up study of human cases with implications for diagnostics. Parasitology Research, 117(12), 3881-3895.

Kolářová L, Matějů J, Hozáková L, Stejskal F, Hrdý J, Kolářová H, Leissová M, Skála V, Dundr P. (2017) Humánní alveolární echinokokóza a přehled výskytu tasemnic Echinococcus multilocularis u zvířat v České republice. Epidemiologie, mikrobiologie, imunologie, 66(4):163-172.

Koucký, M., Malíčková, K., Hrdý, J., Černý, A., Hrbáčková, H., Šimják, P., & Pařízek, A. (2017). The role of maternal imunity and woman´ s microbiome in the pathogenesis of preterm labor. Ceska gynekologie, 82(5), 407-410.

Súkeníková, L., Černý, V., Novotná, O., Petrásková, P., Boráková, K., Kolářová, L., ... & Hrdý, J. (2017). Different capacity of in vitro generated myeloid dendritic cells of newborns of healthy and allergic mothers to respond to probiotic strain E. coli O83: K24: H31. Immunology letters, 189, 82-89.

Medel, S., Syrova, Z., Kovacik, L., Hrdy, J., Hornacek, M., Jager, E., ... & Nyström, B. (2017). Curcumin-bortezomib loaded polymeric nanoparticles for synergistic cancer therapy. European Polymer Journal, 93, 116-131.

Hrdý, J., Novotná, O., Kocourková, I., & Prokešová, L. (2017). The effect of the colostral cells on gene expression of cytokines in cord blood cells. Folia Microbiologica, 62(6), 479-483.

Burns, P., Alard, J., Hrdỳ, J., Boutillier, D., Páez, R., Reinheimer, J., ... & Grangette, C. (2017). Spray-drying process preserves the protective capacity of a breast milk-derived Bifidobacterium lactis strain on acute and chronic colitis in mice. Scientific Reports, 7(1), 1-10.

Chanová, M., & Hrdý, J. (2016). Impact of Induced Th1/Th2 Shift on Trichobilharzia regenti Infection in Mice. Folia Biologica, 62(1), 26.

Hrdý, J., Kocourková, I., Lodinová-Žádníková, R., Kolářová, L., & Prokešová, L. (2016). The effect of a probiotic Escherichia coli strain on regulatory T-cells in six year-old children. Beneficial Microbes, 7(5), 639-648.

Kolářová, L., Matějů, J., Hrdý, J., Kolářová, H., Hozáková, L., Žampachová, V., ... & Stejskal, F. (2015). Human alveolar echinococcosis, Czech republic, 2007–2014. Emerging Infectious Diseases, 21(12), 2263.

Hrdý, J., Novotná, O., Kocourková, I., & Prokesova, L. (2014). Gene expression of subunits of the IL-12 family cytokines in moDCs derived in vitro from the cord blood of children of healthy and allergic mothers. Folia Biologica, 60(2), 74.

Ehrlichova, M., Mohelnikova-Duchonova, B., Hrdy, J., Brynychova, V., Mrhalova, M., Kodet, R., ... & Vaclavikova, R. (2013). The association of taxane resistance genes with the clinical course of ovarian carcinoma. Genomics, 102(2), 96-101.

Hrdý, J., Kocourková, I., & Prokešová, L. (2012). Impaired function of regulatory T cells in cord blood of children of allergic mothers. Clinical & Experimental Immunology, 170(1), 10-17.

Otová, B., Ojima, I., Václavíková, R., Hrdý, J., Ehrlichová, M., Souček, P., ... & Gut, I. (2012). Second-generation taxanes effectively suppress subcutaneous rat lymphoma: role of disposition, transport, metabolism, in vitro potency and expression of angiogenesis genes. Investigational New Drugs, 30(3), 991-1002.

Hrdý, J., Novotná, O., Kocourková, I., & Prokešová, L. (2012). Cytokine expression in the colostral cells of healthy and allergic mothers. Folia Microbiologica, 57(3), 215-219.

Hrdý, J., Zanvit, P., Novotná, O., Kocourková, I., Žižka, J., & Prokešová, L. (2010). Cytokine expression in cord blood cells of children of healthy and allergic mothers. Folia Microbiologica, 55(5), 515-519.

Lodinová-Žádníková, R., Prokešová, L., Kocourková, I., Hrdý, J., & Žižka, J. (2010). Prevention of allergy in infants of allergic mothers by probiotic Escherichia coli. International Archives of Allergy and Immunology, 153(2), 201-206.